Sternvolmer quenching constant value for zniicpc is. The pharmacological actions, such as antiinflammation, antioxidation, antitumor, immunological enhancement, and hepatorenal protection of cphycocyanin cpc from spirulina platensis, have. It may be found in concentrations as high as 7 percent in certain bluegreen algae, as compared to 1 percent chlorophyll content most commonly found. Phycocyanin is believed to support a healthy inflammatory response by inhibiting the key enzyme cox2 that is responsible for causing inflammation and chronic pain. Spirulina is besides used in noodles, fashionable noodles, alimentary, drinks, confects, cookies, natural colouring in masticating. Phycocyanobilin from spirulina the master antioxidant mark f. Supplementing spirulina will provide you with a good amount of phycocyanin, and ill go over exactly how much phycocyanin you can expect to get. Original article phyocyanin extraction from microalgae spirulina platensis assisted by ultrasound irradiation. The possibility that cphycocyanin serves as a nitrogen source in spirulina platensis during nitrogen starvation was studied. Pdf screening for antifungal and antiviral activity of c. One unusual phytonutrient in bluegreen algae such as spirulina is the natural blue protein pigment called phycocyanin. Cphycocyanin cpc could be extracted from cyanobacteria such as spirulina platensis, which. Conformational studies on cphycocyanin from spirulina platensis in. The experimental data on the study of the antibacterial activity of purified phycocyanin, a proteinbound pigment isolated from bluegreen alga, spirulina platensis nordstedt geitler, oscillatoriaceae are generalized and it was shown that phycocyanin was able to markedly inhibit the growth of drug resistant bacteria escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa and.
Pdf stable isolation of phycocyanin from spirulina platensis. Cphycocyanin cpc could be extracted from cyanobacteria such as spirulina platensis, which has been widely used in commercial applications in the food and cosmetic industry as a natural blue dye. The aim of this study was to determine the best method for phycocyaninextraction using three solvents. Phycocyanin in bac enhances the immune system the magic is bac. The discovery of a novel rphycoerythrin from and antartic red. When the supply of nitrogen was low, about 3050% of the cphycocyanin disappeared without any effect. Cphycocyanin as a storage protein in the bluegreen alga. Effects of polysaccharide and phycocyanin from spirulina on peripheral blood. In addition, fluorescence detection of phycocyanin pigments in water samples is a useful method to monitor. Spirulina platensis and its contents have been linked to a nutritional component in both human and animal health and welfare. A food colorant containing phycocyanin from spirulina. Phycocyanin is the most extraordinary component of spirulina. Phycocyanin could be assumed as an antioxidant and has been clinically validated both in vitro and in vivo. Stable isolation of phycocyanin from spirulina platensis.
Spirulina platensis is one of the most famous and popular microalgea that. Spirulina platensis is interesting for the food industry due to its overall composition and high content in c phycocyanin. In most cases, blue hues are based on anthocyanins and do exist primarily in flowers. Cphycocyanin extraction from spirulina platensis wet biomass. Murugan 1 and radhamadhavan 2 1 research scholar, 2 head, dept of microbiology,srm medical college. Microalgae can be adopted as a source of antioxidant. Among many of spirulina bioactive components, blue protein cphycocyanin and its.
Thus, spirulina platensis was recognized as nutritious food by the united nations world food conference. Arthrospira platensis is the widely available source of spirulina that contains distinctive natural pigments, including carotenoids and cphycocyanin cpc. Extraction and purification of cphycocyanin from spirulina platensis. Phycocyanin is a watersoluble blue pigment found only in spirulina and other species of bluegreen microalgae. In this study, flue gas from a power plant smokestack was applied to culture spirulina platensis microalgae. Spirulinas concentrated nutrition makes it an ideal food supplement for people of all ages and. One of the most important requirements to obtain phycobiliproteins from cyanobacterium is optimizing the extraction and purification steps.
Two classes of pigments, carotenoids and cphycocyanin, in spirulina powder. Among many of spirulina bioactive components, blue. Phycocyanin is a pigment protein complex that is heavily sought after for its many health benefits. Natural solutions for blue colors in food sciencedirect. Full factorial design was used to optimise the extraction time. Phycocyanin is the main component in spirulina extract.
Pills and capsules made from spirulina have been used as a addendum nutrient. Optimization,purification and characterization of phycocyanin. Sivasankari, naganandhini and david ravindran department of biology, gandhigram rural institutedeemed university, dindigul, tamilnadu, india corresponding author abstract introduction spirulina is a bluegreen algae owing to the. It contains phycocyanin, a blue pigment, which is known as a nutritious food agent. Phycocyanin powder, broken cell blue spirulina powder. Two classes of pigments, carotenoids and cphycocyanin, in. Extraction and purification of cphycocyanin from spirulina. Spirulina platensis is one of the most famous and popular microalgea that has high protein content and contains much pigments.
Cphycocyanin, a very potent and novel platelet aggregation inhibitor from spirulina platensis. There are two species, arthrospira platensis and arthrospira maxima. Phycocyanins effects are comparable to those of the best known antiinflammatory molecules. Structure of cphycocyanin from spirulina platensis at 2. The taxonomy of spirulina platensis freebooksummary. Instead, phycobiliproteins aggregate to form clusters that adhere to the membrane called phycobilisomes. The experimental data on the study of the antibacterial activity of purified phycocyanin, a proteinbound pigment isolated from bluegreen alga, spirulina platensis nordstedt geitler. Spirulina benefits, nutrition, dosage and side effects dr. Antioxidant activity optimisation of spirulina platensis c. Our results will not only achieve the fixation of carbon. May 06, 2020 blue spirulina contains a bright blue pigment called phycocyanin that is obtained by extracting certain parts from prokaryotes, which is also called bluegreen algae. The commercial production of spirulina has long been implemented in ponds, but.
Spirulina platensis, a multicelluar, photosynthetic prokaryote algae contains a high amount of proteins, vitamins and minerals superior to many foods as e. Spirulina source polysaccharides and immune system. Optimization,purification and characterization of p hycocyanin from spirulina platensis suman saran 1, neha puri 2, nakuleshwar dut jasuja 3, manoj kumar 4 and gaurav sharma 5 1,3,4,5 school of. A method for stably purifying a functional dye, phycocyanin from spirulina platensis was developed by a hexane extraction process combined. Ipi extraction and in vitro antimalarial activity phycocyanin. Phycocyanin is a characteristic light blue color, absorbing orange and red light, particularly near 620 nm, an. One of the active ingredients isolated in spirulina platensis is the pigment phycocyanin, which gives spirulina platensis its characteristic bluegreen color and supplies twenty percent of. Phycocyanin powder, broken cell blue spirulina powder extract. Optimization of phycocyanin extraction from microalgae. Green spirulina has been around for ages and is wellknown throughout the foodie universe. Hsiao g1, chou ph, shen my, chou ds, lin ch, sheu jr.
A novel monoclinic crystal form for phycobiliproteins in phycobilisomes. Spirulina has a documented history of use as a food for more than years, and has been in production as a dietary supplement for 40 years. In this study a simple protocol was developed for purifying phycocyanin pc from spirulina platensis ccc540 by using ammonium sulphate precipitation, followed by a single step. Purification and immunomodulating activity of cphycocyanin from. In vitro antioxidant activities of cphycocyanin extracts obtained from spirulina platensis by freezethaw, microwaveassisted extraction mae and ultrasoundassisted extraction uae methods were. Growth and immunomodulation properties of this supplement have been widely studied in animals and humans, recently. Stable isolation of phycocyanin from spirulina platensis associated. Medical application of spirulina platensis derived cphycocyanin. Although a number of reports are available for the extraction and purification of phycocyanin from cyanobacterial strains schoenleber et al. The crystal structure of cphycocyanin, a lightharvesting phycobiliprotein from cyanobacteria bluegreen algae spirulina platensis has been solved by. Proteins isolated from spirulina have been reported to be quite complex biomacromolecules, likely to be protein andor proteinpigment phycocyanin complexes rather than individual protein molecules. Proteins isolated from spirulina have been reported to be quite complex biomacromolecules, likely to be protein andor proteinpigment phycocyanin. Spirulina and other bluegreen algae contain cphycocyanin, which acts as an accessory pigment when. Study of phycocyanin production from spirulina platensis braz.
The product phycocyanin, produced by aphanizomenon flosaquae and spirulina, is for example used in the food and beverage industry as the natural coloring agent lina blue or exberry shade blue and is found in sweets and ice cream. Bioactivitydirected fractionation of a hot h2o extract from a bluegreenalga spirulina platensis led to the isolation of a novel sulfated polysaccharide named calcium spirulan casp as an antiviral principle. Comparison of different extraction methods for phycocyanin. However, the extraction of phycocyanin from spirulina platensis has not being optimized particularly for its frequency of ultrasound and effect of temperature of extraction to yield. Sivasankari, naganandhini and david ravindran department of biology, gandhigram rural. Jul 16, 2014 spirulina is used as a high quality protein mainly for phycocyanin eriksen 2008, which is a important cyanobacterial accessory pigment having a number of industrial applications. Spirulina denaturation and gel formation is therefore a complex phenomenon chronakis, 2001. Many natural colorants are extracted from microalgea. Influence of thermal and electrical effects of ohmic. B four photobioreactors 50 l tanks with initial volumes of 20 l of s.
Under favourable conditions for growth, cphycocyanin existed in large excess in the algal cells. This was necessary because this dye is known to be very unstable during normal extraction processes. The quality of the water, air, and food in its culture medium are all important for producing good spirulina because impurities or poor quality in the water, air, or food will inevitably find their way into the spirulina itself, with a negative effect on its quality. Antimicrobial activity of spirulina platensis against aquatic bacterial isolates article pdf available in journal of microbiology, biotechnology and food sciences 65. Medical application of spirulina platensis derived c. Phycocyanin is a potent antioxidant and possesses significant immune enhancing and antiviral properties. Novel approaches of using of spirulina platensis in natural rubber based. The best phycocyanin supplement, i present to you spirulina. Oct 05, 2005 c phycocyanin, a very potent and novel platelet aggregation inhibitor from spirulina platensis. Selective inhibition of cyclooxygenase2 by c phycocyanin, a biliprotein from spirulina platensis.
Phycocyanobilin from spirulina the master antioxidant. The following evidence was obtained in support of this idea. In this study, the major carotenoid and cpc contents. Phycocyanin is a potent antioxidant and possesses significant immune enhancing and. Review article medical application of spirulina platensis derived cphycocyanin qianliu, 1 yinghonghuang, 1 ronghuazhang, 1 tiangecai, 2 andyucai 1,3 college of pharmacy, jinan university. Spirulina is grown in alkaline pools and fed with minerals. The interaction of znii ions with cyanobacteria spirulina platensis basic protein cphycocyanin cpc is studied by fluorescence spectroscopy. In vitro antioxidant activities of c phycocyanin extracts obtained from spirulina platensis by freezethaw, microwaveassisted extraction mae and ultrasoundassisted extraction uae methods were evaluated according to 1,1diphenyl2picrylhydrazyl radical scavenging capacity. Spirulina phycobiliproteins as food components and complements. In fruits and vegetables, except for some potato varieties, the shade is more or. Phyocyanin extraction from microalgae spirulina platensis. Crystal structure of a lightharvesting protein cphycocyanin from. The discovery of a novel rphycoerythrin from and antartic red alga.
Extraction and purification of cphycocyanin from dry. The pharmacological actions, such as antiinflammation, antioxidation, antitumor, immunological enhancement, and hepatorenal protection of c phycocyanin cpc from spirulina platensis, have been reported, and cpc has important value of development and utilization either as drug or as functional food. Phycocyanin is biopigment found only in a conductor such as spirulina platensis. In fruits and vegetables, except for some potato varieties, the shade is more or less violet eg, blueberries, purple carrot or black eg, eggplant, tomato and black carrot. In order to extract phycocyanin from spirulina platensis cells, selection of ultrasonic frequency was important. Purified cphycocyanin from spirulina platensis nordstedt. Spirulina phycobiliproteins as food components and. But the best way to find out for yourself is to try a bottle of spirulina tablets or powder and see what it can do for you. Optimization,purification and characterization of p hycocyanin from spirulina platensis suman saran 1, neha puri 2, nakuleshwar dut jasuja 3, manoj kumar 4 and gaurav sharma 5 1,3,4,5 school of sciences, suresh gyan vihar university, mahal jagatpura, jaipur302017,india. Research article phycocyanin extraction from spirulina platensis.
Review article medical application of spirulina platensis. Growth and immunomodulation properties of this supplement have been. All phycobiliproteins are watersoluble, so they cannot exist within the membrane like carotenoids can. Extraction and in vitro antimalarial activity phycocyanin. The commercial production of spirulina has long been implemented in ponds, but photobioreactors and other innovating culture techniques tend to develop. The use of natural colors instead of synthetic colors is of great interests. Yb, a novel synthetic microtubule inhibitor, induces apoptosis of hela. Some of it will be outdated by the time the book reaches the market, but we all hope.
Study of phycocyanin production from spirulina platensis. Phycocyanin is a pigmentprotein complex from the lightharvesting phycobiliprotein family, along with allophycocyanin and phycoerythrin. Abstractthe aim of this work was to study the drying of spirulina platensis, evaluating the final product characteristics about its phycocyanin content and its protein solubility in water. Screening for antifungal and antiviral activity of cphycocyanin from spirulina platensis t.
Quantitative analysis of cphycocyanin from spirulina. Phycocyanin is the most extraordinary component of spirulina the benefits of phycocyanin. This is the pigment that gives bluegreen algae its blue color. Review article medical application of spirulina platensis derived c phycocyanin qianliu, 1 yinghonghuang, 1 ronghuazhang, 1 tiangecai, 2 andyucai 1,3 college of pharmacy, jinan university, guangzhou, china. Blue spirulina contains a bright blue pigment called phycocyanin that is obtained by extracting certain parts from prokaryotes, which is also called bluegreen algae. Spirulina and other bluegreen algae contain cphycocyanin, which acts as an accessory pigment when light energy is captured and transferred to chlorophyll a. Optimization of phycocyanin extraction from spirulina platensis. Quantitative analysis of cphycocyanin from spirulina pacifica low temperature method background. The quality of the water, air, and food in its culture medium are all important for producing good spirulina because impurities or poor quality in. But the best way to find out for yourself is to try a bottle of spirulina tablets or. It is an accessory pigment to chlorophyll and has a characteristic light blue color, absorbing orange and red light. The main pigment of spirulina platensis is phycocyanin, a bright blue color pigment which has many applications in the food industry. Spirulina platensis on skeletal muscle damage under exerciseinduced oxidative stress.
Cphycocyanin, a very potent and novel platelet aggregation. Phycocyanin fromnostoc can inhibit plasmodium falciparum with ic50 8. Screening for antifungal and antiviral activity of c phycocyanin from spirulina platensis t. The purification yield of this method was estimated as 10. Early work done in japan on phycocyanins antiviral properties will be discussed later. Apr 02, 2016 phycocyanin is a pigment protein complex that is heavily sought after for its many health benefits.
Phycocyanin extraction from cyanobacteria spirulina platensis was optimized using factorial design and response surface techniques. Cyanobacteria spirulina platensis basic protein cphycocyanin. Sternvolmer quenching constant value for zniicpc is determined. A method for stably purifying a functional dye, phycocyanin from spirulina platensis was developed by a hexane extraction process combined with high pressure. Spirulina is a simple onecelled microscopic bluegreen algae cyanobacteria. It contains phycocyanin, a blue pigment, which is known as a nutritious food. Comparison of different extraction methods for phycocyanin extraction and yield from spirulina platensis s.